I want to do in vitro experiment to exmaine the role of B cells in tumor invasion by westernblot? Can you recommend some pathways to examine? Thank you very much!
The signal pathways leading to tumor invasion potential depends on cell context such as NFkB signal-induced MMP expression and Rho/ROCK-mediated change in cytoskelton distribution. ECM remodeling and cellular migration occur simultaneously in tumor invasion, so that you should check MMP-2 and -9 expression pattern (i,e, how much the active form is...) and p65 in the nucleus.
I collect the information of tumor invasiveness. It seem to detect EMT pathway(E-cadherin, N-cadherin, vimentin); Adhersion pathway (MPP2, mpp9, Rho/ROCKI,II,VEGF,FAK) matastasis genes(AKT, CXCR4?). Can I try to identify them by qPCR first? Thank you very much!