Which one is best method for constructing a phylogenetic tree among NJ and UPGMA method?

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What can be the reason for DNA extracted from blood samples positive for scrub typhus not coming positive in PCR after 2 months of storage at -20°C?

Good afternoon all. I'm a PhD student from India currently working on scrub typhus disease investigation. I extracted DNA from blood samples using Qiagen blood extraction kit and screened by using...

09 January 2024 6,813 0 View

How to calculate the degree of crystallinity for Polypropylene with multiple melting peaks?

I've been trying to study the degree of crystallinity of Polypropylene using DSC. In the heating curve I find two melting peaks - one at around 150 and the other at 160 degree Celsius. I think...

22 November 2022 5,882 0 View

Culture of Zebrafish spinal cord ependymal cells?

Hi, I am trying to standardize the culture of Zebrafish spinal cord ependymal cells. I dissect the spinal cord. Dissociate the spinal cord by trypsinization and sort the GFP labelled...

30 October 2022 2,260 4 View

Does input concentration affect the dynamic binding capacity of a resin?

Does increasing input concentration causes a decrease or an increase in dynamic binding capacity?

19 February 2020 2,286 4 View

How to run parallel simulations of rigid models with coupled angle constraints using GROMACS-5.1.4?

I want to perform molecular dynamics simulations of a system with toluene solvent represented by TraPPE-UA (7-site) model using GROMACS-5.1.4. It is a rigid model and therefore has no...

02 December 2018 5,120 2 View

How to obtain OPLS United-Atom bonded parameters for Methylcyclohexane?

I want to run a GROMACS MD simulation with Methylcyclohexane (MCH) solvent molecules modeled with OPLS United-Atom (OPLS-UA) force field. I realized that there are only three OPLS-UA types...

23 November 2017 6,346 2 View

How to calculate binding energy between host and guest?

I have one host molecule and one guest molecule. The host molecule contains Zn+2 interacting with four nitrogens that belong to the same host. Initially, negatively charged oxygens each from two...

20 July 2017 7,705 5 View

How to add restriction sites to primers?

i need to clone my PCR fragment into the cloning vector pTZ 57R/T....I am not able to add restriction sites to my primer as i am very new to cloning. Please help me out in detaile how to clone PCR...

13 December 2016 2,046 8 View

How can i find out what would be maximum concentrations of particulate filler could be filled in polymer matrix?

Particulate filled polymer composites are widely available with varied concentrations of fillers. what decides the maximum concentration of the particulate filler? Is there any model where which...

16 November 2016 5,593 5 View

What factor should one consider for developing Health Management Information System?

what factors should one consider before developing HMIS and is there different criteria of developing HMIS at community, district and state and national level, if yes then what are the constant or...

01 September 2015 3,768 5 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?

31 July 2024 5,087 4 View