Can RNApolymerase bind to RNA?

Given that the core enzyme tends to DNA in the cell, because of the electrostatic force. Why is there no such tendency between RNApolymerase 2 and RNA?

14 December 2020 2,708 5 View

How can I insert an assembeled system, meshed by SOLIDWORKS, to ANSYS?

I already did it before but this time as the model is complicated I have faced with tis error: The surface mesh is intersecting or close to intersecting,making it difficult to create a volume...

05 September 2020 9,558 1 View

Is there a way to calculate energies for all residues from a pdb?

CHARMM GUI online does a protein-protein interaction where you can measure the interaction energy of one chain with all the others. Is there a way to do this for each residue? Basically I want to...

29 July 2020 8,851 3 View

Role of intensity in UV-Vis Spectroscopy?

I have two suspensions of Gold Nanorods in Deionized Water synthesized by the same experimental route. Each of the GNR solutions shows the same peak but with different intensities. Is this related...

14 July 2020 9,680 0 View

What are the methods used to amplify the voltage received from two electrodes used in a micro channel to count micro and nano particles?

i'm recently on counting of micro & nano particle by methods like:impedance spectroscopy And I want to get a stronger voltage from my electrodes Who can give me a solution in this matter?

30 June 2020 6,963 8 View

Why would we see generated cracks during heat treatment of the additive manufactured Inconel 625 ?

18 June 2020 2,618 5 View

I want to compare the difference of immune system in patient with mild and sever symptom.who should i define the case and control group?

Is it enough to compare people who needed to be hospitalized with people who did not? , or should the age and duration of the illness be considered?

26 May 2020 2,829 2 View

how can i removed the tackiness of polyborosiloxane (poly borodimethylsiloxane)?

I have synthesized a PBS. this material have a tackiness after synthesized and stick the aluminum foil. how can i removed the tackiness of polyborosiloxane (poly borodimethylsiloxane)?

23 April 2020 2,161 3 View

Why the covid-virus infection is very rare in children compare to young and adult people?

As we know the immune system in both children and adult is impaired. The question is that why the covid-virus infection is very rare in children compare to young and adult people

03 April 2020 9,934 11 View

Is it methodologically true to combine effect sizes in a meta-analysis of clinical trial studies performed on children and adults together?

Recently, I have faced with a meta-analysis of clinical trials which included studies performed on adults and 4-8 years old children and combined the effect sizes of both population groups in the...

24 January 2020 7,571 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

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02 March 2021 3,060 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View