I would like to use B16F0 as feeding cells in a co-culture assay. But, B16F0 are growing so fast that I can not keep them for too long with the others cells that are growing slowly. The medium turn black and the cells lift off the plate at the end. The idea is to stop the proliferation of the B16F0 with the MMC to avoid having overcrowded B16F0 cell population. I tried to treat them with 10µg/ml of MMC for 2h which did not affect their proliferation.
If anybody has ever tried it or has a protocol/suggestion, I will be most grateful.
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