Which methods are useful for fixing mammalian cells for SEM analysis ? Can anybody help me in providing the protocol ?

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I am getting very intense non-specific bands in western blot, but very faint band of protein of interest. What should i do?

I am getting very intense non-specific bands in western blot, but very faint band of protein of interest. What should i do? please see the image and suggest.

06 July 2016 7,944 15 View

Are gold nanoparticles toxic to cancer cells? Also, in which solvent can it be dissolved for cytotoxicity studies?

I have biosynthesized GNPs and wanted to check their cytotoxicity to cancer cells. But after dissolving GNPs in PBS buffer, particles settle down after some time.

31 December 2014 9,903 7 View

I am getting GUS expression in the anther of a plant but I am unable to confirm it by PCR by gene specific primer. Any suggestions?

Transgenics

10 November 2012 2,411 18 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View