As I know it's an expensive pleasure, and there are different methods. But what techniques are cheapest as for insects? I'm a beginner)))
If you can't get DNA extraction kit. You can do a phenol-chloroform extraction. If even that is not an option, you can use physical methods, such as freeze-thaw cycles. All you need to do is take a small amount of tissue homogenate in an appropriate buffer and freeze it to -20 C for 10-20 minutes then thaw it on 70 C for 10-20 minutes and freeze again at -20 C. Repeat this 3 times at least and you might get some DNA out if you are lucky. Another way is to add glass micro-beads to your homogenate and vortex it to break all the cells to release DNA. You can a try combination of these methods. It might work but I do not recommend physical methods because they are not consistent, they are hard to optimize, they may lead to contamination of results during sequencing, plus, I don't think physical methods would be accepted in a good journal. Best to do initial study on crude then get a DNA extraction kit for actual analysis.
Good Luck
If you have only a few samples, you can request a free sample of a DNA extraction kit from a company. They will usually send you enough for 5-10 samples.
Thank you, Hassan and Katie.
Katie, could you tell me about this company?
Regards
If you are not able to buy a DNA extraction kit, I recommend you phenol-chloroform assay.
Also for PCR you can make your Master MIX by yourself. This is the cheapest method.
I think that Salting Out method is very cheap and easy to do.. yo can try,.
Salting out and phenol-cloroform methods are the cheapeast that I know, however it would depend of application for your DNA.
These methods (Salting out and phenol-cloroform) can result in a DNA with some organic solvent or protein contamination, and that can inhibe some polimerase enzymes. If you are going to do sequencing assay I recomend you using a comercial kit that use columns or magnetic beads, in order to get a good quality of your DNA.
I think Salting out method is the cheapest however it has high probability of contamination. Using commercial kit would be better idea.
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