I have extracted collagen and quantified it using Bradford's assay. I tried Lowry's method too, but I got low absorbance values. Bradford's worked better but I still feel there is an underestimation of the actual value. Can anyone suggest a protocol for protein estimation of extracted collagen?
Thank you
A point to watch with Kjeldahl is that depending on other ingredients in the sample, all the N may not be coming from protein. This would result in an overestimate.
https://en.wikipedia.org/wiki/Kjeldahl_method
Bradford method can also give low results if pH is wrong or certain detergents are present.
https://en.wikipedia.org/wiki/Bradford_protein_assay
Use a method that allows the best comparison to other studies unless you are trying to claim the highest level of protein.
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