Parimala Karthik

4 Questions 1 Answers 0 Followers

Questions related from Parimala Karthik

Can I get modified bradford's protocol for estimating total protein content of extracted collagen?

I have extracted collagen and tried estimating total protein content of the same using Lowry's, Bradford's and Biuret's methods. All of them gave low values and I realised these methods will not...

13 March 2023 6,085 1 View

Which method of protein quantification is most suitable for collagen?

I have extracted collagen and quantified it using Bradford's assay. I tried Lowry's method too, but I got low absorbance values. Bradford's worked better but I still feel there is an...

26 December 2022 1,144 2 View

Can anyone share native PAGE protocol for collagen type I detection?

SDS-PAGE details are available for collagen type I and I could get the bands of subunits of the same. I need the protocol for native-PAGE too.

29 November 2022 5,424 1 View

How do I determine the proteolytic activity of 99% pure Papain powder( obtained from Papaya latex)?

I need to use this Papain powder to hydrolyse Collagen once I determine Papain's proteolytic activity.

03 July 2022 637 0 View