Parimala Karthik

3 Questions 1 Answers 0 Followers

Questions related from Parimala Karthik

Which method of protein quantification is most suitable for collagen?

I have extracted collagen and quantified it using Bradford's assay. I tried Lowry's method too, but I got low absorbance values. Bradford's worked better but I still feel there is an...

26 December 2022 1,133 2 View

Can anyone share native PAGE protocol for collagen type I detection?

SDS-PAGE details are available for collagen type I and I could get the bands of subunits of the same. I need the protocol for native-PAGE too.

29 November 2022 5,413 1 View

How do I determine the proteolytic activity of 99% pure Papain powder( obtained from Papaya latex)?

I need to use this Papain powder to hydrolyse Collagen once I determine Papain's proteolytic activity.

03 July 2022 626 0 View