How to work out the concentration for Anti-puromycin diluted by 1:1000?

Hello, I understand I need to use the equation c1v1=c2v2 to work out the concentration of Anti-puromycin 12D10 (0.5mg/ml) diluted by 1:1000 in PBS. However could somebody explain the equation to...

09 October 2017 6,311 3 View

Which method is more accurate and precise from Spot blot and Immunofluorescence?

Which method would detect smaller changes in protein synthesis in a cell?

09 October 2017 6,006 0 View

What scale is integrated density measured in?

I am investigating the puromycin-labelled protein in MSCs. I have taken the integrated density of the immunofluorescence results and Spot blot results. However, what scale do I put for the...

09 October 2017 2,241 1 View

Spot Blot assay result not consistent with immunofluorescence result for same sample?

09 October 2017 7,478 2 View

What is the best statistical test to compare 4 variables?

My project is comparing Serum culturing for 30 and 60 minutes and serum free culturing for 30 and 60 minutes. Therefore I am comparing all 4 variable with 5 ratios for each treatment. What would...

08 September 2017 943 5 View

Does anyone know the best way to compare data of 2 protein synthesis methods- Spot blot and immunofluorescence?

I need to compare data from the spot blot assay and immunofluorescence- (Integrated density from both) to determine the best method of analysing protein synthesis in serum (variable 1) and serum...

08 September 2017 933 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View