Henna Nisar

7 Questions 6 Answers 0 Followers

Questions related from Henna Nisar

What scale is integrated density measured in?

I am investigating the puromycin-labelled protein in MSCs. I have taken the integrated density of the immunofluorescence results and Spot blot results. However, what scale do I put for the...

10 October 2017 2,302 1 View

How to work out the concentration for Anti-puromycin diluted by 1:1000?

Hello, I understand I need to use the equation c1v1=c2v2 to work out the concentration of Anti-puromycin 12D10 (0.5mg/ml) diluted by 1:1000 in PBS. However could somebody explain the equation to...

10 October 2017 6,359 3 View

Which method is more accurate and precise from Spot blot and Immunofluorescence?

Which method would detect smaller changes in protein synthesis in a cell?

10 October 2017 6,082 0 View

Spot Blot assay result not consistent with immunofluorescence result for same sample?

I have attached an image and graphs of the nitrocellulose membrane from the Spot blot assay. My question is regarding the BM8 lanes. From the immunofluorescence analysis it shows that cells in...

10 October 2017 7,613 2 View

What is the reason for better translation of MSCs in serum conditions in culture rather than serum-free?

I am investigating the affects of serum and serum free culture on the translational capacity of MSCs. I have found the MSCs translate better in serum conditions. Why would this be?

09 September 2017 3,921 3 View

What is the best statistical test to compare 4 variables?

My project is comparing Serum culturing for 30 and 60 minutes and serum free culturing for 30 and 60 minutes. Therefore I am comparing all 4 variable with 5 ratios for each treatment. What would...

09 September 2017 1,002 5 View

Does anyone know the best way to compare data of 2 protein synthesis methods- Spot blot and immunofluorescence?

I need to compare data from the spot blot assay and immunofluorescence- (Integrated density from both) to determine the best method of analysing protein synthesis in serum (variable 1) and serum...

09 September 2017 986 2 View