Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

How to plot non-linear kinetics model for column sorption study?

Do I have to solver-add inn for its calculation?

10 December 2020 5,209 7 View

Why many biochar producers find it difficult to sustain in different parts of the world?

Biochar (solid product with more than 60% carbon as per IBI) has been extensively studied for its production, characterization, and applications. In spite of offering numerous benefits in diverse...

23 April 2020 9,534 1 View

Cloning a 4.3kb insert into 6kb vector?

I really need tips for cloning 5kb inserts into 6kb vector. I am trying the cut n paste method using REs. Which ligation ratio should I use? What precautions should I consider? I would be really...

24 November 2019 9,429 2 View

Hii everyone, my spss paired t test value is coming different when i calcualte manually. i have checked many times. which is correct

my sample size is 43. when i am applying in spss its coming 13.5 and manually 0.88, what should i do

31 May 2017 2,409 5 View

How would I do MM-PBSA analysis after MD simulations in GROMACS?

27 January 2016 5,610 9 View

How do I perform Pharmacokinetic study through computational softwares? Is there any online server or any freely available software for it?

I need to perform QSAR and pharmacokinetic study in silico. Please guide me with any server or software which i can freely access and which is quite reliable as per results are concerned.

17 December 2015 301 12 View

How might I calculate Guanidinium concentration in molecular dynamics simulation of proteins?

Hi everyone!! While performing the MD simulations on proteins and studying their unfolding by Guanidium, How should one determine the guanidium concentration with regard to the number of molecules...

29 July 2015 4,437 5 View

Are there any model specification tests with panel data?

Hi I am working on count panel data. Is there any test i can use to test model specification. Linktest and ovtest are not applicable.

23 May 2015 9,295 2 View

Is it possible to study the interaction of a ligand with DNA at different pH conditions through a molecular docking approach?

A ligand showing different effect at different pH. I wanted to study the interaction of the ligand with DNA. So how will i study those at different pH environment through molecular docking...

09 October 2014 5,874 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

How to prepare electrolyte of NaClO4 salt for Na-ion battery? Any problem with this salt?

Hello, I am trying to make anode for Na-ion batteries. I have no experience with preparation of the electrolyte for any batteries. NaClO4 salt are available and I am planning to use EC:PC as...

21 February 2021 8,132 1 View

I want to simulate a system containing protein and heavy metals; which force field is most reliable for this purpose?

The protein I want to simulate has multiple heavy metals. I wanted to know which force-field I should use with GROMACS to achieve this. Most importantly, do the latest updates in the force-field...

16 February 2021 2,420 1 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

By which mechanism does potassium hyrdoxide works as an activator in the preparation of activated carbon?

Activated carbon is prepared from biomass using KOH as the activating agent. What is the possible reaction between KOH and the biomass?

14 February 2021 8,095 4 View

What can be use to mop up heavy metals from vegetables or soils in quarry mining site?

It has been established that quarry mining site polluted and contaminated it's environment with heavy metals, these affects the crops and vegetables planted in the area. The possible solutions to...

12 February 2021 1,155 5 View

Does any one have experience with Autoclavable cell culture media (RPMI 1640/DMEM)? Does it work well?

12 February 2021 2,958 1 View