Which media is best to perform plant tissue culture of bamboo seeds?

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Differences in MALDI TOF mass from the calculate mass?

Molecular weight calculate from primary structure of my protein is 23121 but after MALDI-TOF i got 22908.90 a difference of approximately 213 dalton. Any suggestions for the observed mass difference.

22 October 2018 3,651 3 View

Why cell size increases after transfection with lipofectamine 2000?

When I transfect HeLa cells with lipofectamine 2000, cell size of 10-15% population increases within 48hrs. Transfection works quite well in our lab but I could not see the depletion of the gene....

06 June 2017 1,487 3 View

How to prepare the working stock if the primer concentration in 242.65pm/ul on addition of 200ul water?

18 April 2017 3,317 3 View

Regarding refolding of protein ?

01 January 2017 1,400 12 View

Ion exchange chromatography?

I have performed gel filtration (superdex 75) and anion exchange chromatography for my protein. Solvent used for gel filtration is 20mM PB + 100mM nacl (ph 6.5) and for cation exchange, solvent A...

28 August 2016 3,286 12 View

Suggestions for native gel electrophoresis ?

18 August 2016 2,175 8 View

Can i use Ni-NTA column itself to purify monomers from covalently cross linked dimers. ?

I have a mixture of monomer and dimer. Monomer of the protein has N terminal His tag and i did not remove the his tag after purification. I provide particular condition to my monomers so that two...

20 July 2016 5,347 3 View

Is their any alternative to benzidine chemicals for the staining of peroxidase native PAGE?

To perform a native PAGE assay for peroxidase, what can be the chemical used for its staining?

14 May 2014 4,754 4 View

What is the best percentage of glycerol stock preparation for transformed culture recommended for the long term storage?

What is the best percentage of glycerol stock preparation for transformed culture is recommended for the long term storage? How long will cultures be efficiency maintained?

26 April 2014 9,486 25 View

For Coding sequence trimming which online tool should be used?

To get good phylogenetic tree I need to get some sequences trimmed. I have searched for it but was unable to perform.

07 December 2013 9,398 1 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

No title

02 March 2021 3,060 3 View

Jimmy John Lilly

Check viability of seeds. Seeds that grow in it are viable. If a majority of the seeds are viable then they will grow in MS basal media. Check the agar content of your media. The porosity of agar needs to allow the flow of nutrients to the seed. Kindly check the percentage of agar used. Try using phytagel instead of agar. you can try with chu's N6 media too.