Jimmy John Lilly

11 Questions 18 Answers 0 Followers

Questions related from Jimmy John Lilly

Can we extract toxins from the media or the substrate it is growing in?

Actually these are two separate questions: i. How to extract mycotoxins from the media PDA the fungal culture is growing on? ii. Can we extract the toxin from the natural media it is growing on?

07 September 2020 8,378 5 View

What is the best absorbance value of Agrobacterium culture to be used to infect plant callus?

I need to know the best absorbance value that correlates the growth of Agrobacterium that can be used to infect plant callus. I have tried with 1.0 at 600 nm infection which helped me to transform...

18 February 2018 8,615 2 View

I carried out patchdoc for Protein-DNA and got the result but i need a tool for find interaction energy

I need to find MoleculeInteraction energyVander Waals energyElectrostatic energyHydrogen bond energySolvation free energyInteracting energy I have already tried autodoc since the ligand is a...

11 July 2017 9,164 2 View

Why do I get contaminations after two-three weeks sucessful plant callus growth?

I get contamination either fungal or bacterial after a two week successful establishment of Plant callus. Please tell me why it happens? Endophytes are ruled out totally since even after the...

14 January 2016 2,469 10 View

How can I use the software "Map Inspect" to find locations of genes?

I want to know how to use map Inspect software. I tried searching publications related to Map Inspect protocol. but I was unable to find anything about the same. I need to know the...

08 May 2015 5,907 1 View

What are the new advanced techniques in plant molecular biology involved in proving disease resistance?

Need to know new methods which can help in proving the role of a particular gene in a specific function of the gene.

05 June 2014 6,859 2 View

Can fixing the gel be a problem for no spots in your 2D gel?

I get no spots in my 2D gel. Can not fixing it be an answer? I have tried everything but I cannot seem to fix this problem. Please advise.

22 May 2014 9,095 9 View

Which software or a widely used bioinformatics tool can I use for gene structure?

I need to see the introns and exons of the specific gene sequence of plants. I would like to know how exactly to use them too.

03 March 2014 7,461 2 View

Which one is better Agar-Agar or Phytagel?

How does the gelling medium play a role in supply of nutrients to the ex-plant?

17 October 2013 9,911 12 View

Can we add antibiotics in plant tissue culture media to avoid contamination?

Is there any harmful effect on the growth of the explant ?

28 September 2013 7,365 91 View

What are the disease resistance varieties of tomato against Alternaria solani ?

How does the resistant variety react to a pathogen attack? (in case of tomato: does it produce any specific phytochemical?)

12 September 2013 2,455 5 View