Differences in MALDI TOF mass from the calculate mass?

Molecular weight calculate from primary structure of my protein is 23121 but after MALDI-TOF i got 22908.90 a difference of approximately 213 dalton. Any suggestions for the observed mass difference.

22 October 2018 3,651 3 View

Why cell size increases after transfection with lipofectamine 2000?

When I transfect HeLa cells with lipofectamine 2000, cell size of 10-15% population increases within 48hrs. Transfection works quite well in our lab but I could not see the depletion of the gene....

06 June 2017 1,487 3 View

How to prepare the working stock if the primer concentration in 242.65pm/ul on addition of 200ul water?

18 April 2017 3,317 3 View

Regarding refolding of protein ?

01 January 2017 1,400 12 View

Ion exchange chromatography?

I have performed gel filtration (superdex 75) and anion exchange chromatography for my protein. Solvent used for gel filtration is 20mM PB + 100mM nacl (ph 6.5) and for cation exchange, solvent A...

28 August 2016 3,286 12 View

Suggestions for native gel electrophoresis ?

18 August 2016 2,175 8 View

Can i use Ni-NTA column itself to purify monomers from covalently cross linked dimers. ?

I have a mixture of monomer and dimer. Monomer of the protein has N terminal His tag and i did not remove the his tag after purification. I provide particular condition to my monomers so that two...

20 July 2016 5,347 3 View

Which media is best to perform plant tissue culture of bamboo seeds?

26 April 2016 149 2 View

Is their any alternative to benzidine chemicals for the staining of peroxidase native PAGE?

To perform a native PAGE assay for peroxidase, what can be the chemical used for its staining?

14 May 2014 4,754 4 View

What is the best percentage of glycerol stock preparation for transformed culture recommended for the long term storage?

What is the best percentage of glycerol stock preparation for transformed culture is recommended for the long term storage? How long will cultures be efficiency maintained?

26 April 2014 9,486 25 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View

No title

22 February 2021 9,932 11 View

Can I get information about research laboratories that are currently having in culture, internationally/locally standardised microorganisms?

Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...

18 February 2021 6,500 3 View

Can anyone suggest a software for running a plant tissue culture lab?

A software which can help to design the experiment, store data, update new data, set subculture reminders, barcoding etc. Like Invitrosoft.

17 February 2021 3,015 5 View