I am trying to do some tests to improve the adhesion of HEK 293T cells in T-flask, and I wanted to ask you which matrix do you think is the best to grow them in.
I have the same problem (cell adhesion), but in a little bit different situation. I want to do perform immunofluorescence on a HEK293 cells. The moment they "sense" the glass surface of the slide, and they.. slide away from the glass.
But, the use of gelatin coating on a T-flask can significantly improve the adhesion. The commercial solution is a product from Corning- Cell Bind, but I haven't tried it, yet.