Hi, I need to perform immunofluorescence staining (IF) of primary human keratinocytes, melanocytes and fibroblasts from skin outgrowths. Can somebody advice which marker to use for these 3 cell types and which antibody brand is best ? thank a lot!
Please commend on my theory ? This study begins by identifying a new specific legal issue that intricate into a web of arrogant criminal activities where the crime originates because the key...
25 April 2024 2,930 0 View
What survey platforms work best for research?
17 November 2023 5,075 2 View
I've taken fluorescence microscopy images with 4 channels (DAPI, FITC, Cy3, and Cy5 - Cy5 pseudocolored white) and now I can't figure out how to separate them for analysis on ImageJ or Photoshop....
01 March 2022 4,687 2 View
I am trying to use the ninhydrin assay method to determine hydrolysis degree of whey proteins without nitrogen atmosphere. I used ninhydrin reagent in the ethylene glycol - sodium acetate solvent...
03 January 2021 7,156 2 View
I have a coating with 2 different layers (Al and Zn). After a process under temperature, I want to evaluate the presence of ZnAl2O4 among other oxides (mainly Al2O3 and ZnO). Is possible to...
16 June 2019 9,822 3 View
Is possible to compare crystallinity % by XRD in i-PP (isotactic PP) materials without using a polymer reference? Results could be comparable between them just taking into account the differents...
17 April 2018 9,870 3 View
I am doing a Mass Spec experiment to compare proteomes of healthy Tardigrades and Tardigrades in their "tun" formation. There are several different protocol for desiccating he tardigrades,...
22 March 2018 6,978 0 View
Is it possible to add a DNA sample containing 2 mM DTT in a qPCR reaction with detection with SYBR greenER. Samples are diluted 10x in the PCR master mix which means that the end concentration is...
13 April 2017 5,506 2 View
i am studying the activity of laccase from fungi and I need standard curve of 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) to get the equation Y=aX+b. I have measured the activity of...
23 February 2017 2,850 2 View
Hello, I was looking for an example calculation on how to calculate area per particle (nm^2) after doing Langmuir-Blodgett deposition. For example, if I have spherical silica particles whose...
02 February 2017 5,559 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks
06 August 2024 949 0 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,483 5 View
Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...
25 July 2024 7,520 0 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View
Machine Learning for Automated Diagnosis of Skin Cancer with Dermoscopy Images
21 July 2024 5,470 0 View