Can I stabilize the ninhydrin reagent without nitrogen purge?

I am trying to use the ninhydrin assay method to determine hydrolysis degree of whey proteins without nitrogen atmosphere. I used ninhydrin reagent in the ethylene glycol - sodium acetate solvent...

03 January 2021 6,976 2 View

Presence of ZnAl2O4 by XPS?

I have a coating with 2 different layers (Al and Zn). After a process under temperature, I want to evaluate the presence of ZnAl2O4 among other oxides (mainly Al2O3 and ZnO). Is possible to...

16 June 2019 9,675 3 View

Can you use DTT in a qPCR reaction?

Is it possible to add a DNA sample containing 2 mM DTT in a qPCR reaction with detection with SYBR greenER. Samples are diluted 10x in the PCR master mix which means that the end concentration is...

13 April 2017 5,394 2 View

How can I calculate area per particle ?

02 February 2017 5,444 3 View

How can I clean the silver particles after green synthesis to SEM and TEM?

17 November 2016 477 8 View

How can I measure the concentration of C60 fullerene (nanoparticles) in tissues?

I have exposed clams in vivo to C60 fullerene for 7 days. And I would like to measure a possible accumulation in gills and digestive gland. If you can help me. Thanks in advance.

17 December 2014 5,369 6 View

Can anyone tell me how I can separate anthraquinones?

I`ve been trying separate anthraquinones with no success.

09 January 2014 9,566 3 View

Can anyone tell me if the bacteria inoculated by i.p. are eliminated with urine or feces?

I need to perform a murine sepsis model with P. multocida and M. haemolytica. But for this, I need to know if these bacteria could be eliminated by urine or feces in order to avoid contamination...

04 October 2013 2,285 2 View

Which reliable antibody anti-human cells for cryosections?

Can you recommend me a reliable antibody anti-human cells working for cryosection stainings (IHC-Fr)? We used the anti-NuMa but it does not work properly... Thanks, Marisa

01 January 1970 2,942 4 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View