Can I stabilize the ninhydrin reagent without nitrogen purge?

I am trying to use the ninhydrin assay method to determine hydrolysis degree of whey proteins without nitrogen atmosphere. I used ninhydrin reagent in the ethylene glycol - sodium acetate solvent...

03 January 2021 6,976 2 View

Can I use an Aminex HPX-87H column to analyze sugars in samples with high concentrations of urea?

I have some extracts with sugars in low concentrations, so diluting isn't ideal, and there's about a 17 % of urea (from the solvent). Can the urea damage the column or affect the analysis?

12 September 2019 6,337 1 View

Presence of ZnAl2O4 by XPS?

I have a coating with 2 different layers (Al and Zn). After a process under temperature, I want to evaluate the presence of ZnAl2O4 among other oxides (mainly Al2O3 and ZnO). Is possible to...

05 June 2019 4,129 3 View

According to TEU, the space-time deformation differential within the particles with respect to outer space is what generates what we call mass ?

According to TEU, the space-time deformation differential within the particles with respect to outer space is what generates what we call mass, as well as gravity. Please give us your objetive...

18 December 2018 3,775 3 View

Pcr product below marker?

i have extracted bacterial genomic DNA, and after running multiplex PCR on it, i got blurry thick bands below marker. the marker used is 100 bp. i checked using nanodrop and diluted the DNA conc...

26 April 2018 8,847 7 View

How to calculate crystallinity % by XRD in polymers (i-PP materials) ?

Is possible to compare crystallinity % by XRD in i-PP (isotactic PP) materials without using a polymer reference? Results could be comparable between them just taking into account the differents...

03 April 2018 7,040 4 View

Can you use DTT in a qPCR reaction?

Is it possible to add a DNA sample containing 2 mM DTT in a qPCR reaction with detection with SYBR greenER. Samples are diluted 10x in the PCR master mix which means that the end concentration is...

13 April 2017 5,394 2 View

How can I calculate area per particle ?

02 February 2017 5,444 3 View

Soft agar colony assay: Where should I put my treatment ?

I am performing a soft agar colony assay using 2 different cell lines. Both are able to make colonies. So now I want to test a specific drug and see if it can affect their capability for making...

15 December 2016 4,656 3 View

How can I clean the silver particles after green synthesis to SEM and TEM?

17 November 2016 477 8 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to immerse bricks in wax ( for coating ) ?

Do we immerse the bricks horizontally or vertically ? #bricks#waterproofingsurface#civil#engeenering#chemicalengineering#wax#parafinwax

28 February 2021 6,685 2 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

What pH condition required to coat APTES on silica coated Fe3O4?

It's a hydrolysis reaction, which may produce by either acid or base-catalyzed reaction. so can anybody help me with this, which condition is suitable for this reaction?

26 February 2021 1,760 8 View