Can you use DTT in a qPCR reaction?

More Marisa Vanheusden's questions See All

Can I stabilize the ninhydrin reagent without nitrogen purge?

I am trying to use the ninhydrin assay method to determine hydrolysis degree of whey proteins without nitrogen atmosphere. I used ninhydrin reagent in the ethylene glycol - sodium acetate solvent...

03 January 2021 6,976 2 View

Presence of ZnAl2O4 by XPS?

I have a coating with 2 different layers (Al and Zn). After a process under temperature, I want to evaluate the presence of ZnAl2O4 among other oxides (mainly Al2O3 and ZnO). Is possible to...

16 June 2019 9,675 3 View

How can I calculate area per particle ?

02 February 2017 5,444 3 View

How can I clean the silver particles after green synthesis to SEM and TEM?

17 November 2016 477 8 View

How can I measure the concentration of C60 fullerene (nanoparticles) in tissues?

I have exposed clams in vivo to C60 fullerene for 7 days. And I would like to measure a possible accumulation in gills and digestive gland. If you can help me. Thanks in advance.

17 December 2014 5,369 6 View

Can anyone tell me how I can separate anthraquinones?

I`ve been trying separate anthraquinones with no success.

09 January 2014 9,566 3 View

Can anyone tell me if the bacteria inoculated by i.p. are eliminated with urine or feces?

I need to perform a murine sepsis model with P. multocida and M. haemolytica. But for this, I need to know if these bacteria could be eliminated by urine or feces in order to avoid contamination...

04 October 2013 2,285 2 View

Which reliable antibody anti-human cells for cryosections?

Can you recommend me a reliable antibody anti-human cells working for cryosection stainings (IHC-Fr)? We used the anti-NuMa but it does not work properly... Thanks, Marisa

01 January 1970 2,942 4 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View