Which manual method can be used for accurate measurement of plasma corticosterone level in rats ?

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Adele Romano

We used in our lab the following product

Correlate-EIA™ Corticosterone kit Enzyme Immunoassay Kit

Catalog No. 900-097 Enzo life sciences

For more informations you can ask to Dr. Gaurav Bedse (Sapienza, University of Rome, Italy)

Angela Sarabdjitsingh

In Ron de Kloets lab we routinely used the CORT RIA from MP Biomedicals. We adjusted the standard curve by adding two lower concentrations (6,25 and 12,5 ng/ml) to make sure we would detect low basal circulating levels of corticosterone. Also you just need 5-10 microliters of plasma to run you assay.