How and when Na/K ions get separated from Nucleic acid during DNA extraction?

Na/k acetate is used to neutralize the nucleic acid following alkaline lysis where Na/K binds with the nucleic acid followed by alcohol precipitation. Na/k exist with DNA following elution or we...

04 May 2015 6,453 4 View

Can someone explain why EDTA needs basic condition for dissolving?

EDTA is insoluble in water but dissolve with the increment of pH. Could you please explain how pH works for dissolving EDTA?

08 September 2014 6,873 10 View

How can I load equal volume in each lane during gel running of Western Blot?

07 August 2014 4,787 7 View

How does one calculate protein concentration using formula?

OD is 0.36, what has been got using Lowry method. Is there any formula to calculate protein concentration?

07 August 2014 1,554 29 View

How can you get absolute quantification using standard curve for qRT-PCR?

I have standard curve and know the plasmid size. How can I get the copy number? Can anyone explain details or give reference? It will be really great help.

02 March 2014 4,161 3 View

What is a good Gbs extension?

I have downloaded a mouse chromosome from the NCBI database, which is a gbs file. I opened it in notepad (windows) to see my file, unfortunately all of the document's looking in that file are...

02 March 2014 6,006 2 View

How can I analyse data for absolute quantification, relative quantification and PCR efficiency using standard curve of plasmid?

I am very new in qRT-PCR.

01 February 2014 1,783 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View