S M Majedul Karim

8 Questions 6 Answers 0 Followers

Questions related from S M Majedul Karim

Which loading control is better for brain tissues in western blot?

I need to detect protein in the hypothalamus of brain. As a loading control which one would be better?

07 July 2015 5,328 2 View

How and when Na/K ions get separated from Nucleic acid during DNA extraction?

Na/k acetate is used to neutralize the nucleic acid following alkaline lysis where Na/K binds with the nucleic acid followed by alcohol precipitation. Na/k exist with DNA following elution or we...

05 May 2015 6,523 4 View

Can someone explain why EDTA needs basic condition for dissolving?

EDTA is insoluble in water but dissolve with the increment of pH. Could you please explain how pH works for dissolving EDTA?

09 September 2014 6,938 10 View

How can I load equal volume in each lane during gel running of Western Blot?

For 30 µg protein loading in each lane, concentration of one protein is 1.97 µg/µl and another is 1.41 µg/µl, that means for first one would be 15.2 µl and 2nd one is 21.2 µl. How to load equal...

08 August 2014 4,851 7 View

How does one calculate protein concentration using formula?

OD is 0.36, what has been got using Lowry method. Is there any formula to calculate protein concentration?

08 August 2014 1,592 29 View

What is a good Gbs extension?

I have downloaded a mouse chromosome from the NCBI database, which is a gbs file. I opened it in notepad (windows) to see my file, unfortunately all of the document's looking in that file are...

03 March 2014 6,036 2 View

How can you get absolute quantification using standard curve for qRT-PCR?

I have standard curve and know the plasmid size. How can I get the copy number? Can anyone explain details or give reference? It will be really great help.

03 March 2014 4,220 3 View

How can I analyse data for absolute quantification, relative quantification and PCR efficiency using standard curve of plasmid?

I am very new in qRT-PCR.

02 February 2014 1,819 3 View