There seems to be a variety of E.coli LPS available from companies such as Sigma. I wanted to pulse primary monocytes and T cells with LPS in culture. What should I be looking for when purchasing LPS, i.e method of extraction, species it was generated in? Is there a particular LPS that you have used that you can recommend?
Hi Randeep,
I just briefly wanted to mail concerning your LPS issue. The short answer is that we have always used LPS from E. coli O111:B4, which worked like a charm for B cells, BMDMs and BMDCs.
There is however a paper, which you might want to check, comparing LPS from E. coli 055:B5 and E. coli 0111:B4 not really finding major differences between the two strains for RAW 264.7 macrophage stimulation.
http://www.jbc.org/content/285/38/29375
What surely will play a role is the isolation of the LPS itself, as that will determine the amount of impurities that is contained in your preparation. To minimize lot differences I would suggest to use TC grade LPS isolated by gel filtration- or ion exchange chromatography that contains
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