We are about to recombinantly express a couple of membrane proteins in E. coli and then purify them using detergent-containing buffers. We'd like to first screen a panel of detergents on a small scale so to find out which detergents are more suitable for our proteins. But there are so many membrane protein extraction kits on the market. Can anyone share your experience with any of these kits and give some recommendations? Thanks!
In my own experience, you will need to try quite a few. I worked on membrane proteins with over ten TMD, and tried about 4-5 systems, no luck. Success is probably empirical depending on how nasty the protein is.
We even went out to try weird organisms such as Rhodobacter spheroids still ended up with crapy expression.
Buy some detergents based on the information about the extraction of similar proteins in E.coli.
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