I want to estimate cytokine IL2 , IL4 , TNF , IFN in serum of wistar albino rats.
BD Cytometric Bead Array based cytokine assays may be very useful.
bd biosciences has cytometric bead array kir for cytokine detection in serum it is apromising kit go for it
I have used BD kit and is good one. Inflamation kit will do job for you. But it require FACS. If you dont have one use kits from R&D systems. You get both formats EIA and Chemiluminescence
thanks a lot to everyone, what about Ray biotech kit , it is worthful to do from that bec its little less expensive
Two independent variables 1. Attachment style 1 to 5 likert scale Strongly disagree to Strongly agree 2. Family functioning 1 to 4 likert scale Strongly agree to strongly disagree 3. Childhood...
10 February 2024 6,384 2 View
while using the TRIZOL RNA extraction method. In the precipitation step using isopropanol, polysaccharides like pectin and xyloglucan also get precipitated along with RNA, hindering the following...
30 January 2024 1,390 0 View
1. http://www.libraweb.net/riviste.php?chiave=114 2. http://tbf.peerjournals.net/ Which one is original
26 December 2023 2,961 1 View
Proteins expressed in pPcZaA for protein purification using his trap column in FPLC? how to prepare a sample for that. We have lyophilized the supernatant at 24 hr, 48 hr and run SDS.
25 December 2023 9,012 0 View
please define steps how to upload research paper
27 November 2023 6,287 2 View
Hi everyone, We use DAMASK 3.0. We want to do dwell fatigue simulations. Can we use phenomenological power law for dwell fatigue/creep simulations? Which Model is best for dwell fatigue/creep...
17 October 2023 3,632 0 View
I am trying to validate the detection of plant fungal pathogen through LAMP reaction. But I have an issue with the LAMP reaction. I am detecting the band in NTC (Control) also. To rectify the...
10 October 2023 1,525 2 View
In exothermic reactions, the rate constant decreases on increasing the temperature. When we plot the graph between ln k and 1/T, a positive slope is obtained which gives negative activation...
21 August 2023 372 1 View
How can the Thermogravimetric analysis (TGA) be used to compare similar products?
19 August 2023 3,742 1 View
Could anyone tell how can the TGA (Thermogravimetric analysis) be used to reverse engineer a product?
19 August 2023 9,667 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Dear All I would like to know the minimum number of animals (rats) to be studied for a pilot/preliminary research on intradermal drug delivery. Can I proceed with 1 animal per group for a pilot work?
02 August 2024 4,570 1 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
Dear all, I am working on particle deposition in human's & rat's respiratory airways using CFD and I am looking for the 3D CAD file for my simulations (STEP or IGES format). If somone has such...
29 July 2024 1,092 2 View
i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....
26 July 2024 1,887 0 View
I did an MTT assay with 2 drugs (Piroxicam and Sulfameter) when I got my results for the 12,24,48,72 hours of incubation with my drug at the following concentrations [ug/ml]...
26 July 2024 7,511 3 View