If we want to use an immortalized HUVEC cell line, which one is more preferable to use?
Hello, it depends on what kind of experiments you plan. For instance, SV-40 is not ideal way of immortalization when you want to monitor p53 status because E6/E7 affect the function of p53.
hTERT could be a good choice. But, as Jaromir says, we need to understand what your experiment is trying to achieve.
Immortalization with hTert usually leads to cell lines that closely reflect the "normal" physiology. However, we had some hard times to establish hTert immortalized cells. Therefore we combined hTert with SV40 large T antigen which efficiently immortalized human endothelial cells.
For Tobias: Can you define "normal physiology" in hTERT cell lines. Have you compared cell cyle time, cell adhesion, cell surface proteins before and after immortalization? Just wondering how the cells change.
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