15 Questions 45 Answers 0 Followers
Questions related from Muralidharan Vanuopadath
Hello All, We are using a SCX column to separate crude proteins. After this we are interested in performing in-gel and in-solution digestion of the SCX-resolved proteins. In this case what would...
06 June 2019 8,995 4 View
Dear all, I am wondering why i am getting only less proteins identified from a total protein lysate separated on a 1D gel. Did the in-gel digestion by cutting individual lanes in to 24 bands,...
05 May 2016 9,306 7 View
During sonication/lysis after adding the lysis solution, protein extraction results in foam formation. Does this have any negative impact on protein quality? please provide me with suggestions Thanks,
11 November 2014 9,973 6 View
I run 10% SDS-PAGE gels, at 100V for 2 hours. When I did a 1D SDS gel there were lots of protein bands. However, when I use IPG strips (pH3-10), do IEF overnight and run the gels, no spots...
11 November 2014 7,716 16 View
We precipitated the proteins using methanol-chloroform protocol and redissolved the obtained protein pellet in Tris/PBS buffers. But the proteins are not completely dissolved leaving behind a mass...
11 November 2014 3,973 4 View
We have taken the gel images of a 2D run and was trying to analyze those images in the PD Quest Basic software from Biorad. But the images are not clear when we are cropping it and seems like the...
08 August 2014 3,086 2 View
It seems like there is a frequent change in the pressure when the instrument is in idle condition, without running any samples having an isocratic flow. The column we are using is Poroshell-120,...
07 July 2014 2,701 8 View
We have bought Vitamin K1 and K2 standards from sigma and did the HPLC analysis of K1 by dissolving it in diethyl ether, but we didn't see a peak. We have used Agilent Poroshell-120, 150x2.1mm,...
06 June 2014 5,691 3 View
I have seen a few papers mentioning like they have used urea, chaps, DTT and Pharmalyte for protein extraction. Is pharmalyte an ampholyte? What's the difference between a pharmalyte and an...
05 May 2014 3,754 5 View
What are the components or what lysis buffers can be used to extract proteins from cells so as to perform 2D gel electrophoresis?
05 May 2014 599 15 View
Since suspension cells have to be spun down before removing the media, I am just wondering weather there could be any advantage of using round bottom 96 well plates rather than flat-bottom 96 well...
03 March 2014 5,573 9 View
The cells are procured from ATCC and they recommend to use the specified media supplied by ATCC itself. But since sigma is also providing the same RPMI 1640 media, I thought of going with that....
02 February 2014 1,801 6 View
Being a suspension cell line why do I see some of the cells getting attached to the flasks? Since this is the first time that I am working with these cell lines, can anyone please help me? I am...
02 February 2014 9,170 6 View
If we want to use an immortalized HUVEC cell line, which one is more preferable to use?
12 December 2013 5,295 4 View
Has anyone used immortalized cells for doing experiments and do we have to take care of any specific things while doing/performing experiments with the immortalized cells?
11 November 2013 9,381 17 View
