Which is the best way to desalt proteins purified through Ion Exchange Chromatography?

Hello All, We are using a SCX column to separate crude proteins. After this we are interested in performing in-gel and in-solution digestion of the SCX-resolved proteins. In this case what would...

05 June 2019 8,936 4 View

Why only few proteins identified from a total protein lysate run on a 1D gel subjected to LCMS analysis followed by Mascot search?

Dear all, I am wondering why i am getting only less proteins identified from a total protein lysate separated on a 1D gel. Did the in-gel digestion by cutting individual lanes in to 24 bands,...

04 May 2016 9,243 7 View

Why can't I see any protein spots after a 2D run?

10 November 2014 7,645 16 View

Does the foam formation during protein isolation have any impact on the protein quality?

During sonication/lysis after adding the lysis solution, protein extraction results in foam formation. Does this have any negative impact on protein quality? please provide me with suggestions Thanks,

10 November 2014 9,906 6 View

Why are proteins not fully soluble in the buffer after methanol-chloroform precipitation?

We precipitated the proteins using methanol-chloroform protocol and redissolved the obtained protein pellet in Tris/PBS buffers. But the proteins are not completely dissolved leaving behind a mass...

10 November 2014 3,857 4 View

Can anyone help with 2D gel images seems to be pixelated while cropping the images in PD Quest software?

We have taken the gel images of a 2D run and was trying to analyze those images in the PD Quest Basic software from Biorad. But the images are not clear when we are cropping it and seems like the...

07 August 2014 3,023 2 View

Why do I see a change in pressure when the HPLC instrument is in idle condition?

It seems like there is a frequent change in the pressure when the instrument is in idle condition, without running any samples having an isocratic flow. The column we are using is Poroshell-120,...

06 July 2014 2,635 8 View

What buffers can we use to isolate proteins to perform 2D gel electrophoresis and what is the difference between a pharmalyte and an ampholyte?

I have seen a few papers mentioning like they have used urea, chaps, DTT and Pharmalyte for protein extraction. Is pharmalyte an ampholyte? What's the difference between a pharmalyte and an...

04 May 2014 3,695 5 View

What are the components of a lysis buffer for protein extraction to perfrom 2D gel electrophoresis?

What are the components or what lysis buffers can be used to extract proteins from cells so as to perform 2D gel electrophoresis?

04 May 2014 505 15 View

Which type of 96 well plate is suitable for suspension cells?

Since suspension cells have to be spun down before removing the media, I am just wondering weather there could be any advantage of using round bottom 96 well plates rather than flat-bottom 96 well...

02 March 2014 5,516 9 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View