Depending on the aspect of your research, you might consider fibroblasts (3T3-NIH), macrophages (RAW), chondrocytes, etc. For fibroblasts, it would be more interesting to use primary synovial fibroblasts isolated from RA synovial tissue.
Hi,Koenders,
thank you for your reply ,and it is very uesful for me .
we are just doing some study of methotrexate in rheumatoid arthritis. We are exploring methotrexate mechanism in RA,and finding it difficult to choose macrophages(and which kinds of macrophages )or lymphocytes for the cell line for our work
thank you
we are trying THP-1 for our work ,but We don't know that this choice is correct or not
You could try primary monocytes (e.g. CD14 sorted, or purified by attachment method), further stimulate with M-CSF to differentiate between monocytes and macrophages.
You could use cell lines for mode of action and some basic biology, but to figure out effects in rheumatoid arthritis I guess, you have to go to primary cells and maybe compare treated vs. untreated...
otherwise I agree with Marije on other choices for cells.
Good luck,
Well, you say "exploring methotrexate mechanism in RA", but you have to be warned that MTX would act differently in different cell types (this is related to diffferent proliferative activities, metabolism (including drug resistance) etc. So, whatever cell line would you choose (and I agree that primary cell lines would be best - synoviocytes for example) - your results will be pertinent to the cells of that lineage ONLY, and woud not necessarily explain the overall "methotrexate mechanism".
We have been using primary synoviocytes isolated from RA patients during synovectomy. Upon the tissue culture, both fibroblasts and macrophage-shaped adherent cells started growing together with some suspension cells mostly lymphocytes morphology. However, upon three-five passages, fibroblast cells outgrow and we use them. As compared from fibroblast cell lines such as 293, we noticed different responsiveness to pro inflammatory cytokines such as TNF and IL-1b. Interestingly, when we transformed these rheumatoid fibroblasts by SV40, these responsiveness was very much down-regulated. The cells should be chosen depending on what you want to do. Good luck!
I am not sure if THP-1 would be the right thing for your work.
In our lab we studied effect of methotrexate on RA and Juvenile Idiopathic Arthritis. For this, we always generated fibroblasts from patients' synovial fluid or synovial tissue and used cells between 3-5 passages. While there are several excellent protocols available for the same, we developed ours based on this paper: http://www.jrheum.org/content/32/2/301
Hope this helps- Good Luck!!
I want to use fibroblast primary cell to see the anti-inflammatory effect of hydrogen water in RA patients.
I would like to also know that which cell lines for RA should I used for T cell phenotyping against some medicinal plants having anti-inflammatory property.
- Badges
- Science topic
- Similar topics
- Medicine
- Disease
- Infectious Diseases
- Immunization
- Vaccination
- Vaccines