How can I convert microscopic image length in pixel to um for an image taken from Leica DM2500?

I have been doing work on seedling stomata. the old images cannot be measured in um but can in pixel. so I need to convert the measurement to um.

30 September 2020 7,979 4 View

What are the good and bad things of consuming mass gainer by a male after working out in a Gym?

Some people consumes mass gainer after working out in Gym. What are the good and bad effects of mass gainer?

13 March 2019 5,274 1 View

How can we standardize the concentration of DNA for qPCR?

Srinath Mote

03 January 2017 8,118 3 View

For how long time plant extracts can be stored?

I have prepared some methanol extracts of Rauvolfia root for alkaloid estimation. It is stored in refrigerator and now I want to know the storage life of that extract.

10 March 2016 4,700 10 View

Is there any technique which give instant result about the plasmid transformation in host system?

As we conclude the result of transformation after overnight growth, how could we come over with Instant screening technique.

02 September 2015 2,250 3 View

Do you know a valid questionnaire for socio cultural factors influencing entrepreneurship?

18 August 2015 2,403 10 View

What are the major differences between MDA-MB-231 and MCF-7 cell lines?

Please let me know why MDA-MB-231 cells are more sensitive to any drug treatment when compared to MCF-7 cells? Both of them are human breast cancer cell lines.

08 March 2015 7,189 28 View

Are there any specific primers for the molecular variability study of Rhizoctonia solani 1-1 AG?

19 December 2014 1,064 2 View

How can I get the crystallographic card of Cloisite 93 A ( a modified Montmorillonite nanoclay) & epoxy/cloisite nanocomposites?

Crystallographic card (PDF) JCPDS indexes

14 September 2014 2,036 5 View

Is there a normalization method for implementing PCA and design of classifier for microarray data?

I would like to know which normalization method can be used to implement feature extraction (PCA) and provide it as an input to any classifier. The output of the PCA is not lying in the range 0-1....

26 January 2014 9,318 1 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

How to immerse bricks in wax ( for coating ) ?

Do we immerse the bricks horizontally or vertically ? #bricks#waterproofingsurface#civil#engeenering#chemicalengineering#wax#parafinwax

28 February 2021 6,685 2 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View

What pH condition required to coat APTES on silica coated Fe3O4?

It's a hydrolysis reaction, which may produce by either acid or base-catalyzed reaction. so can anybody help me with this, which condition is suitable for this reaction?

26 February 2021 1,760 8 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How does charge on working electrode changes with rising potential?

During performing CV of a GCE or modified GCE, we rise the potential in the first half of the cycle and reduce it in reverse half. During the first half, what is the nature of charge on GCE or...

25 February 2021 7,994 3 View