within the PCR which test is most appropriate?
For example Allele specific PCR?
hello Sir,
for testing acaricide resistance of field ticks by molecular methods,
Allele specific PCR can be done, it is a good method, through this method you can trace out the genotype present in the population i.e heterozygous or homozygous resistance.
You can go for the normal PCR and sequencing for tracing the mutation. As the mutation for sodium channel in Pyrethroids is only well stated resistance mechanism in India, mutation (C190A) will confirm pyrethroid resistance. In other acacricides mul;tiple mutation has been reported nbut not yet confirmed solidly for resistance.
Sir, Please let me know this helped you and if you need more clarification, you may ask.
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