I need to isolate a specific transcript from cultured cells. Do you have any suggestion on kits or protocols that I can use. I red about streptavidin based protocols.
Do you mean you read ChIRP?
I personally feel that ChIRP is the best way to purify a specific RNA from cells.
You could purify RNA tagged with S1 aptamer, MS2 and Box B using SA, MS2 coat protein and lambda N; but first you have to introduce a tag to your transcript of interest.
No actually I want express a specific gene in a cell line transfected with an expression vector. I need to extract the RNA because I want to evaluate regulatory elements within the sequence and I want to capture this specific RNA in a living cells, because the interaction with ribosomes and proteins is something I want to verify
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