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Questions related from Gianluca Occhi
Has somebody experience in blunting plasmids' sticky ends using a Phusion High Fidelity (NEB)? Suggested protocols?
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Can somebody help me to understand how can I translate a drug dosage used in cultured cells to that that should be used in humans. Does it depend in some way on the molecules I'm working with and...
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I'm planning to perform a clonogenic assay in agar to evaluate the tumor suppressor effect of a gene I'm studying. Going through protocols I see different agar concentrations for top and bottom...
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I need to isolate a specific transcript from cultured cells. Do you have any suggestion on kits or protocols that I can use. I red about streptavidin based protocols.
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Dear all, I am studying a genomic locus in some tumoral specimens with the aim of clarifying if there are differences in the methylation status of about 30 CpGs among different samples (two groups...
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