Hi, now I´m working with a protein wich behaives as a oncogene ( after expression microarrays, functional analysis showed that promotes proliferation, growth and avoid apoptosis). I have some ideas about in vitro studies but, as we know, two heads are better than one. Thanks
Stable overexpression and trying to grow tumour in mice is definitely the best method, but is costly and laborious.
Before, you can get some answer by introducing it to non-tumorigenic cell line and performing colony formation assay or trying to grow them to confluence, to find out if the cells losed the contact inhibition - common feature of cancer cells. You can try also compare expressing and non-expressing cell lines.It will be only circumstantial, but it can give you a hint if it behaves as oncogene and will ease decision to invest more time and money.
Focus formation assay using Colorado serum. Mouse is completely unnecessary.
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