Which is the best protein extraction method followed by 2D analysis in legume plants?

More Bhaskar Chandra Sahoo's questions See All

How to solve 3D tetrahedral traveltime tomography inversion problem?

Hi everyone, I am working on a 3D first-arrival traveltime tomography problem. As part of this, I have the data as first-arrival times (d, 205000), model parameters (m, 170000), and the length...

22 July 2024 8,847 3 View

Any specific peptide based ligand for mitochondrial targeted delivery of nano-drug delivery systems?

Suppose I need to target mitochondria to address malignancy-related problems. Are there peptide-based ligands similar to Nuclear Localization Signals (NLS) for nuclear targeting that can be used...

20 June 2024 9,587 1 View

What is the syntax for non-Schmid coefficients in DAMASK to capture the tension-compression asymmetry?

I have tries "a_nonSchmid_110" or "a_nS" in the material.yaml file, but none of these works. Help is highly appreciated.

27 May 2024 5,545 3 View

How can we predict the PDB structure file for an RNA sequence ? ANy webserver is available to predict the PDB file for a RNA fasta sequence ?

I want to predict analyse the Protein-RNA interaction from modified constructs. For this I have a protein and RNA sequence. I want to see the binding interaction at different positions of RNA and...

12 May 2024 6,795 3 View

Cross-linking density and swelling ratio in the reaction solvent should be provided. What does it mean?

Cross-linking density and swelling ratio in the reaction solvent should be provided. What does it mean? Can you explain?

21 March 2024 5,571 1 View

Why is my sub-cloning not working?

I have two different construct: 1. Insert 1 (~3kb) at XhoI and ApaI site in NCVB vector (9.7 kb). 2. Insert 2 (~1.7kb) at XhoI and ApaI site in in pENTR/D vector (2.6kb) I want to replace Insert 1...

09 March 2024 8,215 7 View

Does sodium level of blood in rheumatoid arthritis patient using methotrexate as DEMARDS falls from the normal value?

It is recently reported as a case study in a medical journal that Sodium level in blood falls down to 119 mEq/L leading to hyponatrenia. Will anybody suggest if it is so what precautions can be...

04 March 2024 2,059 1 View

Can someone please share the CIF file for the MnFe LDH structure?

Dear All I want to explore the properties of manganese-iron layered double hydroxide (LDH) structures and am in need of the CIF (Crystallographic Information File) for the MnFe LDH structure. If...

13 February 2024 2,260 0 View

What can be the suitable media for culturing U-87-MG?

To culture the U-87-MG cell line, various media formulations are available online. These include: a. Essential Minimum Eagle Medium (EMEM) supplemented with 10% fetal bovine serum (FBS), 100 U/mL...

07 January 2024 612 0 View

Anion Exchange Membrane(AEM) available for Non Aqueous Organic Redox flow battery?

I am Sandeep Kumar Sahoo, and I am working on a non-aqueous redox flow battery (NAORFB). As the membrane is an essential component of a redox flow battery, I cannot find a suitable AEM. The AEMs...

04 January 2024 6,606 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?

31 July 2024 5,087 4 View

What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?

31 July 2024 7,921 5 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View