Which is the best option of sample when working with PCR for viruses?

More Leonardo La Serra's questions See All

What human cells lineages are better to use when studying virus (Dengue) infection?

I am currently working with arbovirus evolution in different environment conditions and in different hosts cells. I'm working with dengue virus and in order to study the different hosts I will be...

05 June 2019 7,140 6 View

What is the advantages and disadvantages of using conventional PCR over real time PCR?

I'm currently working with a multiplex PCR for arboviruses detection and I would like to know if that is a better approach than using real time PCR methodology.

08 September 2017 5,629 1 View

Is it possible that organophosphate pesticides induce auto immune responses?

I'm researching the implications of a possible acute or long term exposition to organophosphate pesticides induce to a autoimmune response, due to my recent research try to relate this pesticides...

01 February 2014 4,487 9 View

Does anyone know of studies relating pesticides and heavy metals to the bullous disease pemphigus?

I'm working with the triggering relation between heavy metals and organophosphorus pesticides with the skin disease called pemphigus.

09 October 2013 1,700 15 View

Does anyone know about a methodology to determine organophosphorus pesticides in water matrix by GC- NPD with SPE (cartridges or discs)?

I'll be analyzing river water for organophosphurus pesticides (dichlorvos, diazinon, clorpirifus, parathion, metamidodos and glifosato so far), but I've been having trouble finding a methodology...

07 August 2013 2,050 3 View

Does anyone have statistics to help me to create a control group?

I need to create a representative control group of blood donors to establish the concentration of Pb for my city's population.

07 August 2013 6,026 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Sayan Bhattacharyya

depends on the virus. If Arboviruses are suspected the best sample for PCR is serum within first 5 days. If influenza, mumps, measles are suspected, it will be sputum or BAL. For HIV, it will be blood/serum. For rabies, it is nuchal skin biopsy

Leonardo La Serra

Thank you Sayan. I'm working with Arboviruses, my samples are clotted blood

from deceased primates.

Shiv Sekhar Chatterjee

blood/serum/plasma, csf, brain tissue, any other target organ depending on your virus