Which is the best method to prepare stable copper nano-particles for anti microbial activity applications?

More Akshaya Bhashyam's questions See All

Is the expression of active SARS COV2 of Membrane protein in bacterial system feasible?

I am trying to express the soluble fraction Membrane Protein SARS Cov2 in E. Coli. I am getting only monomers while it's the dimer form that is active. Literature that I have come across have used...

20 March 2023 4,459 1 View

How can dsDNA be visualized with fluorescent dyes in a fluorescent microscope without performing PCR?

Hi, I'm looking for a clean and direct visualization method to image dsDNA (assume purified extract available) with a fluorescence microscope. Currently, the most common ways to "visualize"...

16 January 2023 4,525 1 View

Do we have to remove the "gaps" and "XXXX" after MUSCLE ALIGNMENT in MEGA11?

I'm constructing a phylogenetic tree using microsatellite sequences in MEGA11. I have aligned the sequences using MUSCLE and now I can see a lot of "xxx" and gaps in many of the sequences. Kindly...

13 September 2022 6,517 3 View

What is the best method to extract total proteins from plant tissues ? Kindly suggest with a supporting paper ?

Is using BIO-RAD Total protein extraction kit for plant total protein extraction a good decision ?

26 August 2022 9,344 3 View

If anyone have outsourced your sample for MALDI TOF MS analysis. Can you please share the costing for one sample ?

I'm looking for approximate cost just to include in the funding proposal. Due to short timeline I couldn't get quotation from companies.

17 August 2022 1,004 4 View

How does one create a uniformly thick parabolic reflector in COMSOL?

As shown in the figure, I have a design with rings of a parabolic reflector, each segment with a different thickness. To do this, I created a parabola in one work plane, a circle (guide curve) in...

01 March 2022 4,282 1 View

Biostatistics - How to calculate p value manually in an ANOSIM test?

I just need the explanation or basic examples to show

25 November 2021 1,475 4 View

FMR1 amlification using Triple Primed PCR method not working for full range.Please help?

I have tried amplifying FMR1 using forward, reverse and the chimeric primer . I see a band for 118 repeats. But i don't see any amplification happening for full mutation (645 repeats) and pre...

11 April 2018 5,135 0 View

How to find refractive index of a sample solution if Abbe's refractometer is not available?

When we get capacitance from LCR meter bridge, on substituting values we get dielectric constant of a sample solution. But, how do we get refractive index of the sample solution without using...

30 March 2016 6,130 1 View

Will NLRP3 be secreted in the cell lysate or the supernatant?

For checking the NLRP3 activation in immune cells using Western Blot.

15 September 2015 5,094 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

How to define an anisotropic material with asymmetric elastic compliance/stiffness matrix in ANSYS APDL?

I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...

09 August 2024 5,048 2 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

Binder for ZnO nanoparticles in graphite paste electrode?

I have been using paraffin, but the deposited ZnO still detach from electrode. What is the best binder to modify graphite paste electrode with ZnO nanoparticles?

03 August 2024 4,624 3 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

Mossbauer spectroscopy Co0.6Mn0.4Fe2O4 MNPs?

Following curve related to Co0.6Mn0.4Fe2O4 Magnetic nanoparticles with extra phase a-Fe2O3. I will appreciate to help me to interpretation this spectroscopy, especially the blue line.

31 July 2024 9,140 2 View

Atish Krushnarao Maldhure

Thermal method is easy method to prepare nanomaterial.

Rahim Molaie

I would suggest that the following reference is interesting to your target.

I hope it will be useful for you.

Maurizio Muniz-Miranda

Laser ablation of a copper target in a reducing solution can be used to produce copper nanoparticls