What if top fiber concrete strain exceeds the ultimate strain when fixing the yield strain in steel i.e to calculate yield moment in circular column?

I have prepared an excel sheet to calculate yield moment and curvature for a hollow circular section. In which Depth of Neutral Axis is fixed using "GOAK SEEK" function. Axial Force is input of...

22 November 2020 4,632 3 View

Excel formula for assigning grade?

I have a excel sheet with positive and negative value. I have 4 grades to be assigned. How can I use a formula to assign the grades? I have tried using the IF nested function but due to negative...

21 October 2020 5,648 7 View

How to do MD simulation of docked protein complex after docking .?

I have perform some protein-protein docking studies. I used Hdock, Prizm. But I am unable to find a proper method to verify my docked structure. I also docked my structure in Hex program to get...

19 October 2020 4,083 7 View

Statistical test for Inter and intra rater agreement between 2 observers for gradings? any excel based template for the same?

Hello, I wish to calculate inter and intra rater agreement between 2 observers who are gradings Xrays for degree of bony fusion into 6 grades. In all there are 1750 xrays. Which statistical test...

11 August 2020 1,001 4 View

I want to know about command to fetch XTC and PDB file For markov Calculation?

I am learning markov state model in jupyter notebook. When I am fetching data with mdshare.fetch() command from tutorial its okay. But how I will fetch XTC and PDB File from my local source. Which...

10 August 2020 5,379 3 View

How to fix error of GROMACS topol.top file?

I am doing MD simulations for protein. When i am running the commands the following error is occurred Command which i have used with ubuntu gmx genion -s ions.tpr -o solv_ions.gro -p topol.top...

27 July 2020 3,199 15 View

How common is COVID19 among pediatric population?

Most papers not giving in details about the disease in children. Any one have idea about it. Is Children are protected?

09 March 2020 6,642 39 View

Can a use of Demineralized water with PH of 5 to 6 to clean aluminum cause corrosion?

06 March 2020 793 6 View

How to Manufacture the Nano Powder of Zirconium or Zirconia from Micron Size available in market?

Zirconia or Zirconium NaNo Powder Production

26 November 2019 2,247 3 View

Can someone Help me out with a suitable UDF to import variable properties of SUPERCRITICAL Water?

I am not able to hook up my table of ISOBARIC (225 Bar) PROPERTIES OF SUPERCRITICAL WATER (varying wrt Temp) obtained from NIST into ANSYS FLUENT which only accepts a C Code, Piecewise Linear and...

27 October 2019 4,424 5 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View