Nuclease free water is used for bisulfite sequencing, at stated in this article-https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3214597/
Thank you Sharmin for your answer. But dissolving in TE makes the DNA sample more stable for long term storage. Then why the nuclease free water? Please explain. Thanks
I would also suggest TE buffer because water is disolving CO2 from the air, to form carbonic acid that results in an acid depurination of the DNA and degradation. The TE buffer prevents this.
The EDTA can sometimes interfere in downstream applications, especially for DNA polymerases. But if it's dilute enough, then this shouldn't matter. Storage in a properly sealed container at -20 or -80 is more important.
I agree with Katie. We used the Quick-DNA Miniprep Plus Kit (ZymoResearch) to purify the DNA for our bisulfite sequencing study (doi: https://doi.org/10.1101/239566). They give you the option to elute in their buffer (= TE) or in nuclease-free water (buffered to a pH > 6). So, dissolving your DNA in 10 mM Tris-HCl, pH8 would be a third option, in case you fear EDTA interference.
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