Anti- Covid-19 polyclonal antibodies production?

What are the problems that we might face upon producing polyclonal Covid-19 antibodies using tissue culture?....and if the cured people after been infected with the virus donated their...

07 April 2020 4,152 2 View

What is the most important nutrient element in poultry ratio?

poultry industry

02 March 2019 3,472 4 View

Is there any technique can be used to detect NK cells markers rather than using flow cytometery?

If I cant use flow cytometry, what is the replacement method to be used instead... regards

18 October 2018 2,600 10 View

Is there any technique can be used to detect NK cells markers rather than using flow cytometery?

If I cant use flow cytometry, what is the replacement method to be used instead... regards

09 October 2018 5,005 11 View

What are the most common bacterial pathogens usually isolated from diabetes patients with recurrent UTI?

Most common bacteria isolated in urin samples

07 August 2017 9,803 18 View

Biofilm forming Staphylococcus Aureus, what is the best way to identify this virulence factor?

Virulent Staphylococcus Aureus, pus forming bacteria

06 July 2017 4,675 8 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

No title

02 March 2021 3,060 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

No title

28 February 2021 9,936 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View