I'd like to investigate EpCAM and MUC1 expression in MCF7 and MDAMB231 cell lines by flow cytometry. Which brand of antibody do you recommend?

In addition, I'd like to investigate cytokeratin expression in same breast cancer cell lines but I can't decide to use which cytkokeratin?

17 October 2020 5,794 6 View

What are the growing topics in algebraic combinatorics and graph theory for research?

I need some suggestions what are the growing topics in algebraic combinatorics and graph theory for research? Thank you in advance to everyone who will answer.

21 November 2019 9,552 6 View

What extent reliable are studies done in concrete technology without their segregation stability information?

13 July 2017 4,803 5 View

Which software is better about modeling of self-consolidating concrete to simulate its segregation behaviour

There are some commercial softwares to model the SCC in fresh state in point of segregation with coupled restrainments (aggregate and reinforcing bar) . e.g. Flow3D, Fluent, etc. What are...

29 May 2017 8,476 3 View

Invitation for Journal Reviewer & Section Editor?

29 March 2017 3,388 1 View

Invitation for Journal Reviewer & Section Editor?

29 March 2017 9,285 3 View

H9C2 cells detaching with PBS washing and clamping after tripsin/EDTA treatment. How can I solve these problems?

I m culturing them with DMEM high glucose and % 10 FBS

09 January 2017 7,839 1 View

What kind of downstream analysis should be done on raw NGS data?

12 October 2014 9,899 8 View

Is anyone familiar with silver destain?

I've stained my protein bands with Silver Kit (pierce). It includes a destaining reagent also, but the wash solution which conains NH4HCO3 is not provided. I couldn't find it in our lab. neither...

01 October 2014 369 3 View

What was the linguistic outcome of the Hunnic invasion of the Balkans, especially the Greek Peninsula?

The Huns first entered the Balkans as invaders. Then they started settling in the Balkans. We know they were very influential and active in Pannonia. But we know that they had reached as far as...

10 August 2014 8,343 3 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View