Western blot in brown adipose tissue?

Good morning, I am a PhD student trying to get my head around brown adipose tissue western blot. So far I have successfully isolated proteins with high molecular weight such as PGC1a but I keep...

01 August 2020 8,764 3 View

How big are the neurons in zebrafish? Are there any particular stains useful for label just neurons?

12 September 2017 1,696 3 View

Is there evidence for plasmidic localization of lasB gene in Pseudomonas aeruginosa?

I would like to know if there are evidences for the presence on plasmidic DNA of lasB gene, coding for the virulence factor elastase and references in scientific literature. Thanks

30 January 2015 8,580 1 View

Can anyone suggest me any literature about 2D honeycomb structures?

Can anyone suggest me any literature about 2D honeycomb structures? In particular I am looking for the elasticity tensor which characterizes such materials. Thank you in advance, Sara

11 January 2015 9,448 4 View

Can anyone provide advice on how to purify an S-tagged protein?

I am having trouble purifying my S-tagged protein. All protocols I have read call for batch mode, but say that column mode is also doable. I am using a column with S-protein Agarose from Novagen,...

01 January 2013 5,034 6 View

Does anyone have experience with patients' cell immortalization?

I would like to immortalize blood cells and cells from a skin biopsy. I need advice on which protocol to use.

14 November 2012 4,417 3 View

Does anyone have any suggestions on how to prevent proteolysis during purification of a His-tagged protein?

I am currently in the process of purifying a His-tagged protein. I use a Nickel column and then an ion exchange column to further get rid of other proteins. I have been pretty successful, but I am...

12 August 2012 3,985 24 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

How to modify Johnson cook equation in ABAQUS?

I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...

28 February 2021 4,841 3 View