Looking for external phospholipid bilayer membrane total weight in a human body, not including the intracellular membranes.
Hello. I got a trouble with linking c++ compiler with Abaqus. I installed Visual Studio 2019 first, then installed oneAPI base toolkit and HPC toolkit in order. And I verified link status, C++...
13 May 2024 8,412 3 View
I am asking this as all questions that I can find concern more basic and widespread detachment problems but ours is recent and seems to occur at a specific passage. We have multiple iPS cell...
01 August 2023 2,085 1 View
Hi All I'm a Suicide prevention Outreach Peer Worker and Masters of Social Work Student I'm currently in the process of compiling a literature review am hoping someone might be able to steer me in...
04 June 2023 6,527 1 View
I am optimising the TransIT LT1 reagent for CHO-DP12 cells . I have only achieved an efficacy of 6%. Does anyone have experience with this cell line and reagent and what protocol and efficacy did...
08 March 2023 2,996 1 View
Dear experts, I am running longitudinal models using gamm4 with interest in looking at main effects of group and group by age interactions (age range 8-25 years). I am using the following...
13 February 2023 1,815 0 View
Hi, I have GFP-expressing cells growing in a scaffold that is highly autofluorescent in the green band. Ideally i would like to fix, mount and visualise the GFP within the cells. I know that...
10 January 2023 4,729 2 View
I am trying to identify the exact mechanisms , in which a plasmid get incorporated into the DNA of mammalian cell , during transfection . From literature review I found it is associated with...
11 December 2022 6,626 3 View
Hi everyone! It has been a lot of frustrating days in which I'm trying to fetch this kind of information... I have been told that in a biological system, e.g. a batch bacterial mixed community,...
09 November 2022 2,781 2 View
I am attempting to perform XRD analysis on the 21R Sialon polytype, by seeing how much aluminium nitride is within each sample. Although, I cannot perform Rietveld refinements on this as I have no...
12 October 2022 5,828 4 View
What is the relationship between managers' leadership style and emergency preparedness in Long-Term Care? I am interested in using this RQ for my DBA dissertation. I have been considering several...
05 February 2022 2,960 17 View
Approximate concentrations are require in compared with the WHO permissible limts
11 August 2024 2,723 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View