Which is a classical paper for hybrid modelling in bioprocesses?

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How to label synapses in over-fixed mice brain sections (40 um) via immunohistochemistry (IHC)?

We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...

17 July 2024 7,767 3 View

I see GFP signal on cells even though there is no GFP in them. Can anyone tell me what could be the reason?

These are iPSC derived human cortical organiods and I co-cultured them with tumor cells with GFP expression. After I fixed the tissues and amplified the GFP signal with an antibody. I would expect...

06 June 2024 2,289 3 View

Can anyone help me with GCF (Gingival Crevicular Fluid) elution from PerioPaper?

I am using PerioPpaer from Oraflow, to collect GCF, 8-12 PerioPapers from each subject and a pooled sample in PBS elution buffer. For the elution, I am vortexing for 1 min, then slow...

03 June 2024 9,767 0 View

How to cal culate the direction of dipolemonet for a molecule energy optimized using Gaussian package?

I performed single-point energy optimized using Gaussian software, followed by mapping the electrostatic potential surface around that. There is a clear dipole for that molecule but I want to...

24 May 2024 8,549 1 View

Could anyone kindly provide me the data regarding the variation of water flow rate for 24 hour horizon for small hydro power used in multi period OPF?

Optimal power flow, small hydro

20 May 2024 7,590 0 View

How to calculate the angular velocity of a rotating cell from a microbe video?

I have a video of Brownian motion of microbes under a microscope. From this video, I need to calculate the angular velocity of a particular prominent cell. From my understanding, what I know is...

18 May 2024 4,289 3 View

During ws2 growth via cvd I have noticed black round spots on the si(100) substrate. please suggest me reason behind this?

I am using 20 mg of WO3, 800 mg of Sulphur, 925 oc growth temp, 280 oc S temp, and a 20 sccm gas flow rate.

05 May 2024 8,665 2 View

I have docked 17 antiviral drugs using UCSF Chimera AutoDock Vina. I need to select the best 3. How do I do that?

I need to select 3 best results from the list of the 17 antiviral drugs. How do I select that?

25 April 2024 5,386 1 View

How to detect the polymorphism of a material with monolayer thickness?

We have grown boron nitride with a monolayer thickness. XRD usually is used to understand the polymorphism of any material. However, due to the minimum thickness of the sample, it won't work for...

15 April 2024 5,431 1 View

Under what conditions can Dumitrescu & Hurlin (2012) Granger non-causality test be used?

What is the purpose of using Dumitrescu & Hurlin (2012) Granger non-causality test? Can the test be used for analysing individual cross-sections in a Panel Vector Autoregression model?

05 April 2024 1,031 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View