If a journal doesn't provide doi after acceptance of the manuscript, is it authentic?

It is related to submission of the manuscript in a Journal and the process of its online availability.

18 February 2021 9,269 5 View

How can AI be used for enabling Design for Sustainability?

Design for Sustainability (DfS) is a wicked and complex problem. It needs the designer to combine specialized knowledge from several disciplines with systemic thinking. The designer also needs to...

05 February 2021 2,998 9 View

How to remove colour from DNA pellet during DNA extraction?

I am working with Amaranth accessions which are green to red in color and contain high phenolic compounds. Using the CTAB method with the slight modification I am trying to get high-quality DNA so...

18 January 2021 3,578 1 View

Des anybody have a straight forward protocol for invasion assay using colon cancer stem cells? Is there necessary to dilute the Matrigel?

I am working on colon cancer stem cells to see the impact of combinational therapy.

23 December 2020 9,830 3 View

Is there any software (windows) or spreadsheet except MELTS to understand fractional crystallization behaviour of volcanic rocks (i.e. basalts)?

I have bulk rock major and trace elements data available to me.

17 November 2020 3,531 3 View

Have anyone used galaxy server ?

I have 4 fastq files. From some earlier post I tried to analysethrough Galaxy sever. I used "fastp", "clip adapter" and also "trimomatic" separately three different times to trim adapter. As I...

05 November 2020 1,589 1 View

How to scale an image whose scaling details are unknown?

I want to know how to add scale bar to a histopathological image using ImageJ whose image scale was not recorded at the time of taking the image.I got to know from the technicians that 1micron is...

03 November 2020 10,009 3 View

How to calculate in-plane lattice strain for 2D material?

I am working with h-BN and need to find out the strain due to in-plane lattice constant. The famous Williamson-Hall method may not be applicable here as it analyzes XRD for calculation of strain...

19 October 2020 7,386 4 View

How to do scoring for fat droplets in Histopathological sections?

I want to do scoring for macrovesicles and microvesiclular fat diposits in Histopathological Liver sections. Can anyone suggest me a software and discuss a suitable protocol to achieve this. I...

18 October 2020 9,727 2 View

Significant value post Bonferroni correction?

Hi I did Friedman's RM ANOVA and found data is significant.Then Wilcoxon posthoc analysis is showing significant differences, p=0.001.But Bonferroni adjusted significance is bringing p value to...

07 October 2020 1,262 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View