FAMEs are also series of carboxyl acids with different number of C atoms and hence the retention times. Same is the case with alkanes, just that they do not have esterified carboxyl groups. Kindly clear my confusion.
Thank you!
Hi
a mix of fames(standard) can give you the standard retention times for al the fames. We can easily identify the fatty acid present in any sample with the standard reference retention time.
Thank you Bineesh and Giuseppe. But I am not looking for fatty acids only. My study is untargetted one. I want all sorts of things that can be present in serum of a treated animal.
What kind of analysis you want to perform?
For FAME measurement you need to use FAME standard to make quantative and qualitative analysis. As you mentioned you ate looking for fatty acids so use FAME standard.
Regards
We usually, use the n-alkane series to perform the retention indexes of essential oil components. If you want to investigate the fatty acids, you should use the FAME standard.
Regards
- Badges
- Science topic
- Similar topics
- Physical Sciences
- Materials Science
- Materials
- Polymers