Heena Pahwa

8 Questions 11 Answers 0 Followers

Questions related from Heena Pahwa

Do 1-day protocols for miRNA in situ hybridization work for FFPE tissue sections?

The conventional protocols for miRNA hybridization for FFPE tissue sections stretch for 2-3 days, with prehybridization and an overnight hybridization steps. But some companies like thermo and...

22 June 2022 6,230 0 View

What should be the standard glucose concentration to be used in high glucose studies, that match the physiological levels?

Forgive me for being Naive. I have read that the postprandial concentration normally doesn't go above 140mg/dl, anything above it is considered diabetic. for instance:...

24 July 2019 2,129 4 View

What could be the possible reasons behind the cells dying in the culture plate in few wells while surviving in other, media conditions being same?

I work on MC3T3-E1 cell lines. The cells appear absolutely fine in flasks, but when I seed them in 12/24 well plates for mineralization (nunc thermofischer plates), then cells appear to die in few...

04 February 2019 1,802 3 View

At what stage of the cell cycle do cells arrest during subculturing process? Does the culture become synchronous after few passages?

During animal cell culture, confluent cells are trypsinised, centrifuged, resuspended and finally subcultured. I read somewhere that contact inhibition is used to make the cultures synchronous....

04 February 2019 597 3 View

Can anybody give me the composition of ALP buffer and how much substrate (PNPP) to be added in it?

I need to determine ALP activity in osteoblasts. I want to do it by colorimetric method but the reaction does not seem to work, even after incubating the plate at 37 for 1 hour I am not getting...

13 September 2017 8,515 2 View

How long and under what conditions can I store my derivatized GCMS samples?

I am using MSTFA, O-Methoxyamine in Dry pyridine to derivatize my samples. Usually I try to analyse the samples as soon as possible after derivatizing them. But I want to know whether I can store...

10 July 2017 4,210 5 View

Which is a better retention index standard to be used for GCMS : FAMEs or Series of alkanes?

FAMEs are also series of carboxyl acids with different number of C atoms and hence the retention times. Same is the case with alkanes, just that they do not have esterified carboxyl groups. Kindly...

25 April 2017 7,588 5 View

Can anyone tell me how to proceed for standardization of sonication for getting 200-550bp DNA?

I am trying to standardize chromatin shearing conditions in our lab for doing CHIP.. I am using drosophila wing discs, fix them with 1% formaldehyde for 10 minutes, lyse them and then shear them...

18 November 2012 4,294 8 View