I am working on Plant Fungal Pathogens. I wish to know that after harvesting the fungal spores with 0.1 % Tween 20, how long we can store the spore suspension at 4C for next use ?
For example the viability of zoospores in tween twenty of obligate parasite is around 3 to 4hr, where as conidiospores may stable for a week or months depending on the genus.
you can not keep the spore suspension in the refrigerator because there will be a germination of the spore, you have to store the suspension in the freezer
For long term storage, till 1 or 1 n half year the spores remain viable. Thats my experience. However, for frequent use 1 week to 1 month storage may be fine. However this may differ according to type of fungal culture. I wrkrd with Cladosporium, Asp, Fus, Chaetomella, Colletotrichum, Beltraniella, Alternaria