Hi All,
We want to buy a hypoxia workstation, and prices vary greatly: is it worth to invest more in the "fancy" equipment? Does it perform better? Any feedback from the users? Anyone has experience with Coy equipment?
Looking forward to answers,
Monika
Hey Monika!
I've used the system from INVIVO2 300 https://www.bakerco.com/products/invivo2
It works great, since it lets you incubate your cells and be able to handle them under hypoxic conditions. You can tune O2 levels as low as 0.1% and up to atmospheric. It contain a special door/opening where you can add plates, tubes etc during hypoxia without loss of hypoxic environment.
good luck :)
Hey Monika,
we were using Ruskinn hypoxia station for several years and switched then to Whitley H35 hypoxia station. In my opinion, Whitley chamber is more robust and you have less problems with it. The big disadvantage of Ruskinn chambers are the oxygen sensor which may get wet and you have to replace them then. Now we are using Whitley chamber for something around a year and we had no problems with it until now.
I've used two different Ruskinn/Invivo2 models for several years. It's annoying that you can't clean them with ethanol or a strong oxidizing agent, and we had to modify one of the boxes extensively to make it perform well. If I were buying, I would look for something which is 1) easily cleaned, 2) has foolproof humidity controls and 3) is built using glass and metal rather than plastic and rubber.
Hi All!
I have a new question to you - we got a Coy chamber after all - does anyone have experience with sterilizing it? I cleaned it throroughly with 70% EtOH, put some medium overnight to check, and everything seemed to be infected/grown something in it... How do you keep those things clean?
Thanks a lot,
Monika
BioSpherix has the X3 Hypoxia Hood with separate spaces for high humidity incubation and low-humidity cell handling. It provides full-time oxygen control while reducing contamination risk.http://www.biospherix.com/pdf/x3_configuration_hypoxia%20hood%20v1.0.pdf
Reproducibility within these systems is certainly worth considering. Much of this comes from the measurements these systems use being in percentage as opposed to an absolute measure like mmHg. I did elaborate on this topic in the article below.
https://www.oxford-optronix.com/resources/the-reproducibility-issue-within-hypoxia-chambers-and-a-simple-solution-to-fix-it
The best "hypoxia" workstation is the one that requires no hardware or capital expense. Also one that eliminates the need for gas cylinders and glove boxes, while maintaining perfect physiologic O2 levels and not some random O2 setting on the meter. By the way, the 3% or 5% O2 setting in the internal atmosphere on these systems in meaningless to your cells.
Article Limitations of Oxygen Delivery to Cells in Culture: An Under...
The only thing that matters is pO2 in the media, available to your cells, and no "hypoxia" system regulates the oxygen dissolution in your culture. Check out the Petaka G3 by Celartia. www.celartia.com
You get all of the benefits of oxygen control in a closed system that does not volume due to evaporation, and never needs gas-control, because the microchannel technology auto-regulates to perfect physiological O2 levels. These are cheap to buy and easy to use.
Randy Yerden @ www.BioSpherix.com has been offering in vitro pO₂, sensing, control and data logging for around 10 years. As far as I know it's still available on the Xvivo Systems. It used to be available on the OxyCycler systems as well, probably still is. You need to give them a call, I don't think the upgrade is listed on their site. https://www.biospherix.com/blog/why-should-we-care-about-vitro-pericellular-oxygen
Another problem with hypoxia chambers and workstations. They are not sterile and single-use. They get dirty and you have to find a way to regularly clean and disinfect them to keep them from contaminating your samples. Good point.
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