How can I do a wound healing assay under hypoxia conditions, without interrupting hypoxia?

More Arnau Calvet Carnice's questions See All

Sample size to estimate a Cohen's kappa index?

I would like to know the way to calculate the sample size of a study to determine the agreement (weighted Cohen's Kappa index) between two thecniques.

11 February 2018 6,936 4 View

How can I calculate the sample size for the cross-cultural adaptation of a questionnaire?

Questionnaire - Cruciate Ligament-Return to Sport after Injury (ACl-RSI). 12 items

14 February 2016 8,249 4 View

Why do my fibroblasts under hypoxia conditions proliferate slower than the same fibroblasts under normoxic conditions?

I am using healthy control lung fibroblasts in a 96 hour experiment with two drugs (nintedanib and Pirfenidone) and vehicle controls for both. I have both hypoxia (approx 1% O2) and normoxia...

12 January 2021 6,260 1 View

Is there any chemical or surgical method to induce hypoxia in mouse model?

Dear all, My question is about Inducible hypoxia in vivo (mouse model). I'm looking for some chemicals that can induce the pathway of hypoxia or some other techniques. If you now know something...

15 December 2020 866 6 View

Why the western blot of HIF1alpha of the whole cell lysate shows intense band under normoxia without any induction of hypoxia?

I am working with oral cancer cell lines and trying to find the expressional changes of HIF1alpha under different experimental treatment. I am getting intense band of HIF1alpha in the western blot...

29 November 2020 5,707 3 View

RIPA content? any additional suggestion?

i am working on phosphorylation of NF-kB inducible in hypoxia using MDA-MB 231 cells. i have challenges getting my phosphoproteins through western blot. this is my RIPA content 50 mM Tris pH...

13 November 2020 3,356 3 View

Can we detect neuroprotection, ischemic tolerance, ischemic pre conditioning using fmri ?

I want to know whether there are any non invasive techniques to detect neuroprotection in humans

02 October 2020 5,896 3 View

ELISA of isomer of glycolytic intermediate 2,3 DPG ?

Hello, I recently performed Kit based ELISA for 2,3 DPG on plasma samples of control and hypoxia treated rats. [CAT. NO. E1562Ra, Bioassay technology laboratory] However, the results I obtained...

07 September 2020 3,270 2 View

Which one is causing stress in rice, aerobic or anaerobic conditions? or both?

We know from the literature that rice plant has evolved different mechanisms to tolerate hypoxic stress. However, as rice normally grows under hypoxic conditions (during germination and seedling...

05 September 2020 8,220 5 View

What are some in vitro experiments that can be performed to target hypoxia?

I want to show that my developed nanoparticle targets hypoxia but from doing research it seems like most hypoxic targeting related experiments are done in vivo. I'd like to be doing in-vitro...

16 August 2020 3,695 4 View

Can hypoxic air (1% O2, 5% CO2, 94% N2) buyed from the market?

I need hypoxic air (1% O2, 5% CO2, 94% N2) for hypoxia experiments, can I get/buy some hypoxic air from the market?

14 July 2020 5,471 2 View

What is the best stock concentration and final concentration of Cobalt 2 chloride hexahydrate?

Hello Everyone, I have some questions related to a drug called Cobalt 2 chloride hexahydrate. I am currently preparing an experiment related to Hypoxia. I bought a drug called " Cobalt 2...

10 May 2020 4,841 4 View