Are there papers on?

I'm looking for a papers concerning either Christianity or neopagan religions and social media. What role does social media play in the spread of these religious movements? Any suggestions on the...

15 February 2021 655 4 View

Which commercial DNAse do you recommend me?

Which is the best DNAse (or DNAse kit) you have worked with? I am looking for a good DNAse that can remove DNA from an RNA sample in one pass. Material would be used for future RT-qPCR analysis....

18 November 2020 8,769 3 View

How to extract big peripheral membrane proteins?

I have been trying for a long time to see by Western Blot a 450 kDa-peripheral membrane protein from the Golgi apparatus with no success. I know that the electrophoresis and the membrane transfer...

28 September 2020 9,664 3 View

Maiden vs married name?

When I claimed authorship of papers written under my maiden name, they have now appeared under my married name, and I am worried this will cause confusion. How do I fix this issue?

12 August 2020 6,936 5 View

Rat DRG survival issue - how can I debug the protocol?

Hello everyone, I have been trying to establish primary rat DRG neuron culture for a couple of months already. I am using the protocol I found somewhere during the Google search, apparently...

14 June 2020 343 1 View

How to clean analytical size exclusion column?

Hello, We are using Superdex75 increase 3.2/30 GL size exclusion column in our lab and recently column pressure is to high to work on recommended flow rate 0.1 mL/min. I tried to clean the column...

19 May 2020 8,549 4 View

Unexpected problems running Photoquad software: How to fix the issue when it doesn't run anymore?

Hi, I've been using Photoquad software to analyze photoquadrats but suddenly the program doesn't run anymore and I am not able to solve the problem. I uninstalled both the MATLAB Compiler...

21 April 2020 4,707 1 View

Did any of you use polyethylene glycol to break down antibodies in Western Blot?

Hi, I've got a problem with my Western Blot. I have a low weight protein to detect but I suspect that my primary and secondary antibodies are clumping into a large ball at a higher molar mass than...

09 March 2020 2,856 3 View

Can anyone tell me the results of European Xenome research?

I know that the European Union has funded XENOME, an integrated project within the Sixth European Framework Programme (Life Sciences, Genomics and Biotechnology for Health), lasting 5 years...

18 November 2019 6,532 2 View

Hi all, is it possible to use XBridge BEH Amide Column to separate acetoin and 2,3-butanediol via HPLC?

Most of the methods that I have found recommend Aminex HPX-87H but I would rather avoind buying new column. Thanks, Marta

13 November 2019 6,221 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

No title

03 March 2021 5,360 2 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Sample size determination for non-parametric tests?

How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...

24 February 2021 166 6 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View