Can anybody suggest a positive control for detecting phospho specific AKT(Ser473) in Flow Cytometry?

18 April 2024 0 4K Report

I want to check the phospho-AKT level in T cells by Flow . So, i am looking for a good positive control.

Thank you

Badges
Science topic

More Marta Vadori's questions See All

Can I change concentration and brand of antibodies mid-experiment?

I am doing immunohistochemistry of the c-Fos protein and I have a budget problem. For context, the experiment has been done over 3 years prior to my arrival and I have 40 brains done and 100 left....

29 July 2024 5,961 2 View

Paired t-test or unpaired t-test for my quantitative data with SPSS?

I am conducting a qualitative-driven approach to mixed-method research. The role of my quantitative data is to corroborate the findings of the qualitative data. Qualitative data has been collected...

24 July 2024 9,799 3 View

Have you combined pragmatism and interpretivism before?

Has anyone combined pragmatism and interpretivism in their research? If so, could you please recommend some reading to help with this? I am currently writing the methodological chapter for my PhD...

21 May 2024 2,639 2 View

¿Está la escuela española preparada para que se lleve a cabo la inclusión real de todo el alumnado?

En el informe TALIS 2018: Estudio Internacional de la enseñanza y aprendizaje dirigido a la mejora de la formación y el ejercicio docente, se han incluido aspectos relacionados con la innovación y...

11 May 2024 5,550 0 View

What is the best medium for Caco-2 cell line?

Dear Researchers, I would like to start working with Caco-2. I have seen in articles that different media (e.g. MEM or DMEM with various suplementation) are used to culture Caco-2. Which is the...

29 April 2024 4,949 2 View

A good book about disturbance ecology?

Hi I am trying to find a good, classical book or review about disturbance ecology. Any advice would be very helpful. Thanks in advance!

16 April 2024 4,641 1 View

The black pellets after protein precipitation?

Good morning, I used TCA/aceton protocol to precipitate proteins. I incubated vesicles from yeast with TCA. After centrifugation 14000xg, 10min at 4°C I saw black pellets in each samples... What...

21 March 2024 1,670 4 View

¿Cómo puedo informar correctamente aceerca de un posible caso de trata?

Todo el mundo alega que si ocurriese una injusticia lo correcto sería contarlo pero, cómo se debería dar voz de forma adecuada en este caso, sin poner en riesgo a las víctimas?

10 March 2024 5,202 1 View

¿Es lo mismo trata de personas que tráfico de personas?

Normalmente se suelen utilizar esos dos términos coloquialmente a la hora de hablar sobre el tema, y me gustaría saber las diferencias para llevar el tema con propiedad y que más personas sean...

10 March 2024 2,567 1 View

It is correct to use GAPDH as housekeeping gene in cells with a mitochondrial disorder?

I understand the importance of choosing a good gene standard for normalization of qPCR values. Even so, I would like to know if it could be performed (unless it is not the best option) the...

06 March 2024 4,638 1 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 639 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

How to do Mann-Whitney U test with Bonferroni corrected p-values?

Dear All, My lab primarily works on insect wing patterns. In one of the projects, my student and I have defined 19 abnormality characters on the forewing and 6 abnormality characters on the...

31 July 2024 6,464 5 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,623 1 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Bonferroni correction. I have independent t-test, paired t-test and ancova conducted. Which test would require Bonferroni adjustment?

I have two groups that I test on three different tasks. I have 4 independent t-test, 6 paired t-test and 2 ANCOVA. My concern for which t-test should I conduct bonferroni correction. At the moment...

28 July 2024 7,827 6 View

Can I use Likert scale with Paired Sample T-test?

Hey researchers! I am currently doing a research about to what extent in which A has accelerated the inclusiveness of the payment system in my country. Down below are a few examples of the...

26 July 2024 5,654 3 View