Which glycosyltransferase mediates glycosylation of collagen protein ?_Which glycosyltransferase mediates glycosylation of collagen protein ?
Which glycosyltransferase mediates glycosylation of collagen protein ?
I'm doing in-vitro drug release study using dialysis bag method and collecting samples from the external medium at several time points, subjecting to UV-Vis spectrophotometry. But my absorbance...
14 July 2024 6,829 2 View
I suspect my cells are contaminated with mycoplasma. I fixed the cells with 4% PFA and stained them with DAPI. Below is the image I obtained. I don't observe the typical small, rounded DAPI foci...
11 July 2024 7,786 3 View
There are black dots that seem like contamination in my corneal endothelial cell cultures. Some of the cells appear unhealthy and are enlarged. The black dots are more prevalent around these...
11 July 2024 4,829 0 View
Dear colleagues, As well known, we can deliver messages in different languages and characters, including Chinese, English, Latin, binary and decimal codes (if with a converter) and many other...
08 July 2024 5,422 13 View
I did linear regression of X (independent variable) to M (Mediator) then I used survival regression to fit X to Y (dependent variable) With these questions: a. HOW to correctly do a mediation...
17 June 2024 1,478 6 View
The protein was expressed using the insect cell system, and the recombinant plasmid was successfully constructed as verified by sequencing, and the recombinant plasmid was transformed into DH10...
13 June 2024 2,590 4 View
Hi, I am very new to Bioinformatics. Recently, I have the project that aims to perform taxonomic anlysis on raw reads from mixed samples taken from environment. For example, we perfromed NGS...
09 June 2024 6,537 3 View
Hi, I have a question regarding western blot. I performed total protein statin after trasfering membrane. The result was totally fine before (Figure 1). However, there were strange bubble-like...
30 May 2024 8,045 3 View
I am wondering if the Gamma function is completely monotone within the interval (0,1). I use Mathematica to plot the derivatives of Gamma function up to 10th order of derivatives and the results...
29 May 2024 2,846 3 View
I want to know what the 50 mcm screen exactly is, is it a kind of filter?
28 May 2024 1,437 0 View
The NS1 protein of the tick-borne encephalitis virus potentially has three glycosylation sites. When processing the PNGase F protein on SDS-PAGE, I observe three bands - a fully glycosylated 52...
01 August 2024 3,519 4 View
Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you
24 July 2024 3,937 0 View
Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...
19 July 2024 3,906 0 View
I am intrested in the preparation of collagen carriers, dehydration, paraffin infiltration, and paraffin embedding. Thanks in advance.
05 June 2024 3,831 0 View
Previously I have used GlyProt to add carbohydrate chains with my modelled protein, but the server is not working now. Is there any alternative tool for modeling glycosylated protein?
01 June 2024 894 1 View
I have been making hydrogels regularly using 10X DMEM (phenol red indicator), Ultrapure water, 1N NaOH, and 3.41 mg/ml Collagen (suspended with acetic acid). We have used the same calculations for...
29 May 2024 4,655 0 View
Can rats that were injected with collagenase NPs, show higher collagen deposition than their control untreated counterparts in histopathology examination!!!
11 May 2024 2,931 0 View
I made a myocardial tissue using cardiomyocytes and type I collagen (3 mg/ml). During the culture process, the tissue remodeled and its width decreased. What factors influence the degree of...
10 May 2024 7,784 0 View
hello, I have a scaffold with three layers, two synthetic polymer layers and one central layer made of ECM gel seeded with SMCs. After in vivo culture, how can I determine through Masson's...
05 May 2024 2,297 0 View
how to extract collagen from bones specimen?
27 April 2024 6,307 0 View